Live cell microscopy: From image to insight

Live-cell microscopy is a powerful tool that can reveal cellular behavior as well the underlying molecular processes. A key advantage of by visualizing biological processes, it provide direct insights. Nevertheless, live-cell imaging be technically challenging and prone to artifacts. For successful experiment, many careful decisions are required at all steps from hardware selection downstream image analysis. Facing these questions particularly intimidating due requirement for expertise in multiple disciplines, ranging optics, biophysics, programming cell biology. In this review, we aim summarize points need considered when setting up analyzing experiment. While put particular focus on yeast, concepts discussed applicable also other organisms. addition, discuss reporting data sharing strategies think critical improve reproducibility field.